IN-VITRO STUDIES ON OIL DEGRADING BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL OF VANIYAMBADI AND AMBUR AREAS OF VELLORE DISTRICTAbstract
The oil polluted soil has many hydrocarbons which can be degraded by microbes. These oil degrading microbes were isolated from the oil contaminated soil through plate and dilution technique. The oil contaminated soils were collected from the oil spilled places of Vaniyambadi and Ambur areas of Vellore District, Tamil nadu, India. Through the biochemical tests, the isolated bacterial strains were identified as Pseudomonas aeruginosa and Bacillus subtilis, the strains were further confirmed by starch hydrolysis test and by growing on cetrimide agar. It is screened for the biosurfactant production through drop collapsing test and Emulsification Index (E24) using diesel, petrol, kerosene and tween 20. Biosurfactants are amphiphilic compounds which reduce surface and interfacial tension. Here, Pseudomonas aeruginosa had shown higher biosurfactant activity, when compared to Bacillus subtilis.