IN-VITRO TRANSPORT EVALUATION OF GARCINIA MANGOSTANA L. PERICARP EXTRACT LOADED CREAM AND GEL

Garcinia mangostana L. pericarp (GMP) has been known as a traditional medicine in Asian countries. The bioactive from GMP has been recognized to be related to complex phenolic compounds linked with free radical scavenging activity. This research aimed to study the effects of topical formulation types (gel, oil in water cream and water in oil cream) of GMP extract on the in-vitro release and in-vitro shed snake skin penetration. The formulations were evaluated for viscosity and pH. The extent of GMP extracts released and penetrated shed snake skin were evaluated based on stable radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and expressed by Radical Scavenging activity equivalent GMP Extract concentration (RSGMPE). Mangostin skin penetration was quantified using UV spectrophotometric method. GMP extract exhibited significantly higher DPPH scavenging activity compared to α-mangostin which is known as the major constituent in the GMP extract. RSGMPE released and penetrated through the skin was significantly influenced by the type of formulations. GMP extract loaded gel provided highest RSGMPE amount transported through the skin than that retained in the shed snake skin membrane. On the contrary, total mangostin was found more retained in the shed snake skin membrane. When loaded in the gel, pure α-mangostin showed similar in-vitro shed snake skin penetration profile. GMP extract is potential to be developed used for topical applications with suitable formulation strategy for skin targetting.