IN-VIVO ANTI-ULCER SCREENING OF METHANOLIC EXTRACT OF AMARANTHUS SPINOSUS

Background and Aim: The Amaranthaceae family have gained attention for their potential antiulcer properties. Peptic ulcers, a prevalent gastrointestinal disorder, necessitate the exploration of alternative therapies, and Amaranthaceae species may offer promising leads in this regard. Therefore, we have selected Amaranthus spinosus from the Amaranthaceae family for the evaluation of antioxidant and antiulcer properties. Method: The plant material was successively extracted by the Soxhlet extraction technique with Petroleum ether, chloroform, methanol, and water. The antioxidant activity of the plant extracts and the standard drugs were based on consideration of the radical scavenging influence of the stable “1,1-diphenyl-2-picrylhydrazyl” (DPPH) free radical action and hydrogen peroxide process. Two models, and non-steroidal anti-inflammatory induced ulcer and pyloric ligation, were analysed for methanolic extract of Amaranthus spinosus for its antiulcer activity. Result: The phytochemical examination of methanolic extracts reveals the presence of triterpenoids, flavonoids, alkaloids, tannins, glycosides, and proteins. The antioxidant study results revealed that among all the four extracts, the MEAS possesses significant antioxidant potential. Compared with control, there were a significant (p<0.05) decrease in gastric volume, total and free acidity in the extract-treated classes and standard class. The MEAS revealed significant (p<0.05) percent inhibition, i.e. 59.42 and 69.59 percent with 500 mg mL-1 PL and NSAID, respectively. Conclusion: The present research findings demonstrated that the methanolic extract of Amaranthus spinosus possesses significant antioxidant and antiulcer activity.