IN-VIVO ANTICANCER ACTIVITY OF TABEBUIA ROSEA (BERTOL) DC. LEAVES ON DALTON’S ASCETIC LYMPHOMA IN MICE

The purpose of this study was to investigate the experimentally possible antitumor effect and antioxidant role of methanolic extract of Tabebuia rosea leaves against Dalton’s ascetic lymphoma (DAL) induced Swiss albino mice. The methanolic extract of Tabebuia rosea (METR) was administered at the doses of 100mg/kg, 200mg/kg in mice for 14 days after 24hours of DAL inoculation. The effects of METR on the growth of bodyweight, MST, viable and nonviable cell count, Tumor volume, Packed Cell Volume (PCV), life span of DAL bearing mice were studied. Hematological profile and liver biochemical parameters (lipid per oxidation, antioxidant enzymes) were also estimated. Treatment with METR decreased the tumor volume and viable cell count thereby increasing the lifespan of DAL bearing mice. Alteration in the Hematological parameters, protein, PCV occurred because of DLA inoculation, were almost restored in METR treated groups. The effect of METR also decreases the levels of lipid peroxidation and increases the levels of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT). The histopathological results showed the loss of liver hepatocytes in DLA bearing mice. However, a mouse treated with METR extract improved the liver and rearranged more or less normal architecture. The present work revealed that the METR exhibits significant antitumor and anti-oxidant activity in vivo. These results support the traditional use of Tabebuia rosea and the alcoholic seed extract is a potent source of anticancer compounds that could be utilized pharmaceutically.