IN-VIVO ANTIHYPERLIPIDEMIC ACTIVITY AND PRELIMINARY PHYTOCHEMICAL SCREENING OF BAUHINIA ACUMINATAAbstract
The global burden of CVD is rising due to its increasing prevalence in lower- and middle-income countries where 80% of all global CVD-related deaths occur. Common significant risk factors for dyslipidemia included obesity, diabetes, and dysglycemia. The present study was aimed to investigate the potential antihyperlipidemic activity of ethanolic leaf extract of Bauhinia acuminata in atherogenic diet-induced rats. Preliminary phytochemical screening showed the presence of flavonoids, alkaloids, steroids, terpenoids. Acute toxicity study was performed on the rats using OECD Guideline no. 423 and the maximum safe dose was found to be 5000 mg/kg. Animals were grouped into normal control (Group-I), disease control (Group-II), standard group (Group-III) received Standard drug Atorvastatin 10 mg/kg, and test group (Group-IV) received ethanolic extract of the plant with doses 200 mg/kg and 400 mg/kg. The study was conducted for 30 days and hyperlipidemia induced using atherogenic diet. Statistical analysis was performed using Graph Pad Prism and the values are expressed as mean ± SD values. Bodyweight changes were observed every day upon Induction of Hyperlipidemia and it was drastically reduced in the rats with 400 mg/kg dose (190 ± 10) compared with the standard group (180 ± 18.27). There is a significant reduction in the total cholesterol, VLDL, triglycerides, and increase in HDL level along with reduction in random blood Glucose level with ethanolic extract at a dose of 400 mg/kg then 200 mg/kg and the standard atorvastatin. Atherogenic index of the plant extracts 400 mg/kg (1.62) 200 mg/kg (4.69) also compared with normal group (1.37), disease control group (10) and standard group (3.11).
A. Govindula *, M. S. Reddy, M. Manjula, M. S. Reddy, Nusrath and P. Kalyani
Department of Pharmacology, Vaageswari College of Pharmacy, Beside LMD Police Station, Thimmapur, Karimnagar, Telangana, India.
20 April 2019
20 August 2019
01 September 2019
01 February 2020