IN-VIVO ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT FROM ROOTS OF SMILAX CHINA ON ALUMINIUM CHLORIDE INDUCED OXIDATIVE STRESS IN WISTAR RATSAbstract
To examine the antioxidant property of ethanolic extract of roots of Smilax china (EESC) on Aluminium Chloride Induced oxidative stress in Wistar rats. The phytoconstituents are extracted from the roots of S. china using ethanol, by hot continuous percolation method. The rats were divided into 5 groups (6 animals/ group) and treated with EESC (150 & 300 mg/kg of body weight) and piracetam (0.5 mg/kg of body weight) for 14 days after inducing oxidative stress with AlCl3 (100 mg/ kg of body weight) for 60 days. The lipid peroxidation level (TBARS) and antioxidants activities like Superoxide dismutase (SOD), Catalase (CAT), and Glutathione (GSH) were estimated. When compared with the control group, AlCl3 induced rats showed increased TBARS and decreased antioxidant enzymes like Catalase (CAT), Superoxide dismutase (SOD) and reduced glutathione (GSH). The EESC at higher dose 300 mg/kg of body weight animals were significantly (P<0.001) reduced the TBARS and increased the antioxidant enzymes SOD, CAT, and increased GSH when compared with the control group. The present study results revealed that the ethanolic extract from Smilax china roots might be used as a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses and oxidative stress-related diseases.
B. Sabarisenthil *, A. Yokeshwaran and V. K. Kalaichelvan
Department of Pharmacy, FEAT, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.
22 October 2019
26 March 2020
28 March 2020
01 October 2020