IN – VIVO CONFIRMATION OF A 49.6 KDA PROTEIN PILI OF HELICOBACTER PYLORI TO PREVENT DESTRUCTION OF GASTRIC CELLS AGAINST LIVE HOMOLOGOUS BACTERIA IN MICEAbstract
Peptic ulcers is one of the major gastrointestinal disorder in human being that generally associated with the infection of Helicobacter pylori, a gram-negative microaerophilic bacterium in stomach. It is also linked to the development of the stomach cancer. The aim of this study was to investigate the in vivo properties of the sub-unit pili proteins with molecular weight of about 49,6 kDa in mice. The bacterium was firstly cultured on the plate of TSA-B (Trypticase Soy Agar with 5% Sheep Blood) to prepare the protein of interes using bacterial cutter and SDS-PAGE. The purified protein was used for a vaccine emulsified with commercial cholera toxin and give orally. The immunized mice showed a significant protection against challenge with live H. pylori cells. In contrast, animals that received the 49, 6 kDa protein without adjuvant as well as the negative control with PBS failed to inhibit adherence of the bacteria, as indicated by a severe damages of gastric tissues. This study has indicated that the sub-unit pili proteins trigered the release of protective antibodies againts the microorganism if combined with cholere toxin adjuvant. Further study is required to investigate the biological functions of this protein as a vaccine candidate for protecting the infection by this microorganism in causing gastric ulcers.
Hamong Suharsono , Sumarno Reto Prawiro *, I Nyoman Mantik and Made Agus Hendrayana
Laboratory of Microbiology, Medical Faculty of Brawijaya University Malang, Indonesia
21 July, 2014
29 September, 2014
01 December, 2014
01 March, 2015