IN-VIVO PRELIMINARY EXAMINATION OF MORINGA OLEIFERA LEAVES EXTRACT AS ANTIAGING CANDIDATE IN SWISS WEBSTER MALE MICE (MUS MUSCULUS)

Free radicals are molecules with an unpaired electron that very unstable and react quickly with other compound to gain stability. Once the process is started, it can be a cascade reaction resulting disruption of a living cell. The free radicals formed in our body are combated by antioxidants then interact with free radicals and terminate the chain reaction before vital molecules are damaged. Moringa oleifera leaf extract well known can be as a source of antioxidant since it contains flavanoids for reducing free radical. This study was design to produce a crude extract of dark green and bright green of M. oleifera leaves using different solvent to determine its antiaging effect in male mice Mus musculus Swiss Webster. The antioxidant capacity of the extract were carried out using TMAMQ (Tetramethoxy Azobizmethylene Quinon) and ABTS (2, 2-Azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) method, while the antiaging activity were assessed by histological approach. The results showed that the highest antioxidant capacity was achieved by dark green M. oleifera leaves ethanolic extract followed by double-destilated water (ddH₂0) and 95% n-hexane extracts. The antiaging activity were observed by level of M. musculus skin dermis thickness as 516.13 μm and more fibroblast cells 115.67 cells in the visual area of 157.883, 92 μm² of skin dermis. The result indicated that the colour of M. oleifera leaves and type of extraction solvent play important roles on the antioxidant capacity and antiaging activity.