IN VIVO TOXICOLOGICAL EVALUATION OF CHLORPYRIFOS PESTICIDE ON FEMALE ALBINO MICE: THERAPEUTIC EFFECTS OF CURCUMA LONGAAbstract
Chlorpyrifos (CPF) an Organophosphate insecticide was evaluated for its toxicity to produce reproductive disorders in rats following oral exposure. In the present study CPF was given to the mice at the dose levels of 10 and 20mg/kg body weight by Gavage method. A histological alteration of ovary and uterus was examined under light microscope. Its oxidative stress levels were studied by noting lipid peroxidation levels and hormonal changes (FSH) were also checked to evaluate the fertility rate in CPF induces toxicity mice. LD50 CPF was established for Swiss albino mice. Bioremediation was done to toxic induced mice using crude and extracted curcumin at 100 and 200 mg/kg body weight respectively. In control group, the germinal epithelium was continuous with prominent and well defined different stages of graffian follicles. On the other hand chlorpyrifos (CPF) treated group showed ruptured germinal epithelium with multiple nuclei and matured graffian follicles with degenerated ovum. Corpus leutium cells were observed to be rudimentary. Unusual number of vacuolated spaces was observed in ovarian cortex. Thus, the present study reveals that chlorpyrifos causes degeneration of graffian follicle and germinal epithelium of ovary leading infertility in female. Control uterus shows well defined longitudinal and circular muscles on periphery with well structured endometrial cells and glands. In case of drug treated, degeneration of circular and longitudinal muscles was observed on periphery with clustered endothelial cells at lower concentration. At higher concentrations, degeneration in circular muscles is prominent. Longitudinal muscles were also on periphery with many vacuolated spaces along with vacuolated endometrial cells and glands were also rudimentary in structure. The remediation effect with crude and curcumin it showed the better and satisfactory results in histopathology as well as biochemical aspects.
K. Madhavi and V. Sai Saraswathi*
Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore, Tamil Nadu, India
11 September, 2010
25 November, 2010
23 January, 2010
01 February, 2011