IN VIVO TOXICOLOGICAL EVALUATION OF ORGANOPHOSPHATE PESTICIDE ON FEMALE ALBINO MICE: THERAPEUTIC EFFECTS OF CURCUMINAbstract
Chlorpyrifos (CPF) an Organophosphate insecticide was evaluated for its potential to produce reproductive toxicity in rats following oral exposure. In the present study mice were given CPF at the dose level of 10 mg/kg body weight by Gavage method for 14 and 21 days. Morphological alterations of ovary were examined under LM. Its oxidative stress levels were also evaluated by Lipid peroxidation levels and hormonal changes (FSH) were also measured to evaluate the fertility rate in case of CPF induced toxicity mice. Bioremediation was done to the toxicity induced mice using extracted curcumin at 100 mg/kg body weight for 7 days. In control group, the germinal epithelium was continuous with prominent and well defined different stages of graffian follicles. While in chlorpyrifos treated group ruptured germinal epithelium with multiple nuclei were observed. Degenerated ovum was observed in matured graffian follicles. Rudimentary cells were observed in corpus leutium. Many vacuolated spaces were observed in ovarian cortex which is unusual. Significant increase in serum LPO levels and decrease in FSH levels were also observed. After treating the toxic mice with the curcumin restoration of all the parameters to the normal status was observed.
K. Madhavi and Ranjit Kumar*
Research Section, Mahavir Cancer Sansthan and Research Center, Phulwarisharif, Patna, Bihar, India
23 May, 2010
19 August, 2010
03 September, 2010
15 September, 2010