INHIBITION KINETICS OF E. COLI BY METHANOLIC ROOT EXTRACT OF ALSTONIA SCHOLARISAbstract
The potential of methanol extract as a potent antimicrobial agent has been observed to be an effective means to combat disease causing pathogenic organisms. Growth pattern of Escherichia coli cells showed that the growth of cells decreased with an increase in concentration of extract. The specific relationship between the specific growth rate and the substrate concentration was thus be obtained by fitting these data into Monod kinetic model. The non competitive and uncompetitive inhibition kinetic models were also tested in order to determine the inhibitory effects of the methanol extract of roots on Escherichia coli. Longest lag phase of 8 hrs was obtained at nutrient broth concentration of 2 g/L. Kinetic studies showed that with an increase in concentration of extract (10-30 g/L) the specific growth rate and inhibition constant decreased but saturation constant increased. Therefore the biokinetic constant estimated by these models have laid the possibility of obtaining suitable antibiotic from Alstonia scholaris.
Pratyush Kumar, C. S. Misra, Joel James, Lipin Dev M. S., Arun Kumar Thaliyil Veettil and Thankamani V*
School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
08 July, 2011
05 August, 2011
17 October, 2011
01 November, 2011