INVESTIGATION OF IN-VITRO THROMBOLYTIC AND IN-VIVO ANTIPYRETIC, ANTIDEPRESSANT POTENTIALITY AND ANALGESIC ACTIVITY WITH PHYTO-CHEMICAL NATURE OF METHANOLIC EXTRACT OF NYMPHOIDES HYDROPHYLLA

Nymphoides hydrophylla (NH) is mainly an aquatic herb of Menynthaceae family and traditionally used to treat fever, insect bites, different ulcers, and also to wash skin, where parasitic affection occurs. In this study, methanolic extract of Nymphoides hydrophylla (NH) plant was examined for the determination of thrombolytic, antipyretic, Central Nervous System (CNS) and analgesic activities. The evaluation of thrombolytic potential, antipyretic, CNS and analgesic activities of the plant extract occurred by their clot lysis, temperature reducing, and depression-inducing and pain sensation decreasing ability respectively. The result from the study showed the thrombolytic activity 3.05 ± 0.70% to 26.45 ± 2.18% indicating the better thrombolytic activity at a lower dose. The maximum pyrexia suppression of 91.07% is observed for 400 mg/kg NH extract. In hole cross and open field tests, maximum 71.42% and 66.80% suppression of locomotor activity were observed with the methanolic NH extract (400 mg/kg) whereas suppression of the locomotor activity of the standard drug diazepam was 68.57% and 51.20% respectively. From the analgesic data, about 91.72% inhibition was observed for 400 mg/kg of NH extract. Statistical calculation was carried out by using one-way ANOVA followed by Dennett’s multiple comparisons tests, where statistically significant values from control *P<0.05, **P<0.01, ***P<0.001. Finally, this investigation indicates that due to having a potent bioactive compound in case of CNS disorder and pain sensation, N. hydrophylla could be a natural alternative medication of depressant and analgesic agent.