INVESTIGATION OF SELECTED HERBOMINERAL FORMULATION T-AYU-H & T-AYU-HM™ PREMIUM AS AN ANTISICKLING AGENT FOR SICKLE CELL ANEMIA

Sickle cell anaemia is a genetic blood disorder that is caused by a mutation in the gene that encoded b-globin of haemoglobin, which results as the presence of abnormal haemoglobin HbS in the RBC. Presently, there is no universal cure for sickle cell anaemia Herbomineral formulations were standardized for organoleptic properties (colour, odour and taste), physiochemical properties, total ash value, moisture content, pH of the solution, to find  effective agent in which we investigated the antisickling potential of T-AYU- HM Premium, a novel Herbomineral formulation. In-vitro sickling of homozygous sickle cells upon deoxygenation (Emmel’s Test) showed dose dependent inhibition of sickling in presence. T-AYU-H and T-AYU-HM Premium (25 µg/ml, 50 µg/ml, 100 µg/ml and 500 µg/ml). Mechanistic evaluations were performed such as, t-BOOH induced oxidative haemolysis, and formation of met-haemoglobin was significantly decreased in dose dependent manner in the presence of 50 µg/ml, 100 µg/ml and 500 µg/ml of T-AYU-H and T-AYU-HM Premium. Membrane stabilization activity by osmotic fragility test showed significant dose dependent increased stabilization in the presence of 50 µg/ml, 100 µg/ml and 500 µg/ml of T-AYU-H and T-AYU-HM Premium. The T-AYU-H and T-AYU-HM Premium will provide improvement of the therapeutic profile and lesser side effects compare to available synthetic treatment such as Hydroxyurea and Decitabine, and chances of blood transfusions will also be decreased. Formulations are much more affordable compared to available cost of management of disease for sickle cell anemic patients.