ISOLATION AND ANTIMICROBIAL AND DEGRADATIVE POTENTIAL OF ACTINOMYCETESAbstract
Problem Statement: Do the soil Actinomycetes do have Antimicrobial and Petrol degradation potential? An intriguing question. Actinomycetes are continues to be a subject of study with reference to their Antimicrobial and degradative potential. However studies have been done is limited. Our object was to study its Antimicrobial activity in wide spectrum and to study its degradation potential on Petrol.
Approach: In this study we have isolated total 5 Actinomycetes from the Ganga river bed. All the isolates later purified and identified by various Morphological and Biochemical test. Here Nocardia was subjected to antimicrobial test against Streptococcus, Mucor and Aspergillus and it was also subjected to degradation test against Petrol.
Result: The 5 isolates are Streptomyces, Micromonospora, Micromono sporangium and 2 different strain of Nocardia (Na1 and Na2). The 2 strains of Nocardia are active against Streptococcus (Na1 29.6mm, Na2 26.6mm), Mucor (Na1 12.5mm, Na2 22.5mm) and Aspergillus (Na1 50%, Na2 60%). They also degrade Petrol very effectively, decrease in total organic carbon of the medium was observed during the degradation of petrol.
Conclusion: Our observation provides us with evidence that these agents can be used for the production of new antibiotics and as the agent to control the environment pollution.
Padma Singh* and Vani Sharma
Department of Microbiology, Girls Campus Gurukul Kangri (Deemed University), Hardwar- 249 407, Uttarakhand, India
18 October, 2012
07 January, 2013
29 January, 2013
01 February, 2013