ISOLATION AND CHARACTERIZATION OF LEAF SPOT PLANT PATHOGENS FROM SPINACIA OLERACEAAbstract
Spinacia oleracea L. (child spinach) is a generally new leaf vegetable in the Amaranthaceae family. The crop is high in human supplements and generally has significant degrees of bioactive mixtures like nutrients A also C and minerals. In the present study, the fungi and bacteria from the infected leaves of Spinacia oleracea are isolated and morphological identified. DNA isolation of the cultured fungi and bacteria was done. The isolated genomic DNA was amplified using the internal transcribed spacer region and 16s rRNA region. The new DNA sequences were identified at the genus and species level using the BLAST software tool (NCBI, USA), deposited in (The National Center for Biotechnology Information) NCBI, and got Genbank accession numbers. The DNA sequences that showed maximum similarity to query sequences were selected and analyzed using MEGA 11.0 (Molecular Evolutionary Genetics Analysis) for phylogenetic analysis. The phylogenetic tree was generated based on the Neighbour-joining method.