ISOLATION AND CHEMICAL CHARACTERIZATION OF POTENTIAL BIOACTIVE COMPOUNDS FROM ALBIZIA STIPULATA BARK

The present research effort was undertaken to isolate phytoconstituents from pharmacologically active extracts of stem bark of Albizia stipulata based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of bark of Albizia stipulata were prepared using various solvents such as methanol, hydro alcohol, and water. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. The active extract was subjected to column chromatography through a mixture of solvents to get fractions and eluted fractions were run in TLC using various mobile phases with different solvent ratios. The isolated compounds were subjected to IR, 1H NMR, 13CNMR and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of bark of Albizia stipulata was potent compared to other extracts. This extract was subjected to column chromatography to get fractions, and eluted fractions were run in TLC. The fractions with similar R_f values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be β-Sitosteryl-3-O-β-D-glucoside and 3 -O methyl D- Chiro Inositol. Conclusion: Various extracts from the bark of the plant Albizia stipulata were prepared. Β-sitosteryl-3-O-β-D-glucoside and 3-O methyl D-Chiro Inositol were isolated from the methanol extract of bark and characterized.