ISOLATION AND CHEMICAL CHARACTERIZATION OF POTENTIAL BIOACTIVE COMPOUNDS FROM CASSIA UNIFLORA

Objective: The present study was designed for Isolation of phytoconstituents from pharmacologically potent extracts of leaves of Cassia uniflora based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of leaves, stems, and fruits of cassia uniflora were prepared using various solvents such as water, methanol and hydro alcohol. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. These active extracts were subjected to column chromatography by means of different mobile phases followed by TLC. The isolated compounds were subjected to IR, ¹H NMR, ¹³C NMR, and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of leaf of Cassia uniflora was found to be potent when compared with other extracts. This extract was subjected to column chromatography to get fractions and eluted fractions were run in TLC mobile phase with different solvent ratios. The fractions with similar R_f values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be Methyl inositol, Luteolin, pentacosane, and Triacontan-1-ol. Conclusion: various extracts from different parts of the plant of Cassia uniflora were prepared. Methyl inositol, Luteolin, pentacosane, and Triacontan-1-ol were isolated from the methanol extract of leaves and characterized.