ISOLATION AND IDENTIFICATION OF THE FLAVONOID “QUERCETIN” FROM TRIDAX PROCUMBENS LINN.Abstract
Tridax procumbens (Linn.) is an important medicinal plant belonging to the family Asteraceae. It is known for a number of pharmacological activities like antidiabetic, anticancer, anti-inflammatory, wound healing, hepatoprotective and antioxidant activity.Flavonoid “Quercetin” was isolated from the flowers of this plant. Dried flowers were Soxhlet with petroleum ether, chloroform, and methanol successively. The methanolic fraction so obtained was successively extracted with petroleum ether, diethyl ether and ethyl acetate. The ethyl acetate fraction was hydrolyzed with 7% H2SO4 and extracted with ethyl acetate to obtain crude Quercetin. The crude product was recrystallized with dilute ethanol to get pure Quercetin. The purified material was subjected to various chromatographic and spectral techniques such as UV, IR, HPTLC, HPLC, NMR etc. and was identified as “Quercetin”. This study is also of practical importance because quercetin is an important constituent of Tridax procumbens and it has many uses such as in cancer, diabetes, inflammation, & in viral infections etc.
Nikita Sanghavi, Rashmi Srivastava and Yashwant Malode*
MET Institute of Pharmacy, Bhujbal Knowledge Center, MET Complex, Bandra (W), Mumbai 400050, Maharashtra, India
12 November, 2013
12 January, 2014
23 March, 2014