ISOLATION AND PURIFICATION OF NIMBIDIN FROM AZADIRACHTA INDICA OF WESTERN GHATS AND EVALUATE ITS ANTIOXIDANT, ANTI-BACTERIAL AND ANTI-DIABETIC PROPERTIES

Azadirachta Indica has always been known to be the bestowed with good health for many centuries in India. It has been found that the Neem is native to Asian countries. There are three major active components in A. indica, namely nimbin, nimbidin, and nimbiene. The compound nimbidin was isolated by chromatographic techniques. A. indica leaves extract was prepared, and the phytochemical analysis was conducted, and the phytochemical screening manifested the presence of alkaloids, flavonoids, reducing sugar, phenol, saponins and cardiac glycoside in the neem extract. Then qualitative phytochemical analysis was conducted for flavonoids, alkaloids, saponins, and phenol. Plant sources are considered the major active source of antioxidant molecules for a long time in the history of humankind. In the current study, the anti-bacterial properties of Neem have been tested against gram-positive bacteria, namely, Staphylococcus aureus, Bacillus subtilis, and gram-negative bacteria, namely, Escherichia coli Pseudomonas aeruginosa, Klebsiella pneumoniae, and proteus vulgaris. Chloramphenicol was used as a standard to analyze the effect of the nimbidin compound. The compounds obtained from plants are mostly categorized by their antioxidant activity. DPPH free radical scavenging activity and Nitric oxide scavenging activity has been performed in our study to determine antioxidant activity of A. indica. Diabetes results from an unusual metabolism of insulin. Azadirachta indica plays an important role in management of Type II Diabetic Mellitus by improvising the insulin action and secretion.