ISOLATION AND STRUCTURE ELUCIDATION OF BIO-ACTIVE COMPOUNDS FROM BOMBAX CEIBA LEAF EXTRACTAbstract
Bombax ceiba is a traditional medicinal plant that is beneficial in the treatment of diuretic, hemostatic, antidysenteric, emetic, antipyretic, astringent, and commonly known silk cotton tree, semal, kapok tree. The aim of this study is to isolate and characterize the biologically active compounds from the ethanolic extract of the leaves. The EtOH extract was subjected to silica gel (60-120 mesh) by glass column chromatography, using gradient petroleum-ether, C6H6, CH3OAc, CH3OH, and purified by preparative TLC (GF254) to yield two pure natural products (taraxerol and β-sitosterol glucoside). The structure was elucidated on the basis of spectroscopic analysis, including 1HNMR, 13CNMR, DEPT-135, COSY, HSQC, and HMBC. The β-sitosterol glucoside shows pharmacological activities such as lowering cholesterol levels, reducing blood pressure, antitumor activity, etc. The glucose moiety is highly effective because it is hydrophilic, which inhibits the entry of cholesterol into the esterification of cholesterol. It is the first time isolated from the leaves of this plant.
M. Rehan, Shafiullah * and O. Singh
Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
31 July 2019
26 November 2019
18 April 2020
01 July 2020