ISOLATION, CHARACTERIZATION AND PHYTOCHEMICAL EVALUATION OF ACTIVE COMPOUND THYMOL FROM CYCLEA PELTATA LAM

Cyclea peltata (Lam), belonging to the family Menispermaceae, is a common plant in South India. It is mentioned in Ayurvedic classics by Rajapatha and is one of the important drugs used in Ayurveda therapeutics and is used widely in different formulations in medical practice. The quality control of crude herbal drugs and their bio constituents is paramount in justifying their acceptability. The crude drugs can be identified systematically on the basis of their morphological, histological, chemical, physical and biological studies. Phytochemical screening reported the presence of alkaloids, carbohydrates, glycosides, phyto sterols compounds and proteins and amino acids. The plant is widely used in the treatment of cough, fever, kidney disorder, urinary disorder and snake poisoning. The aim of the present study is to extract and isolation of bioactive compound (Thymol) from Cyclea peltate (Lam) Hook f. Thoms, the isolation and characterization analysis, included Thin Layer Chromatography, High-Performance Thin Layer Chromatography, GC-MS and Spectroscopy studies (IR, NMR and MASS). The presence of thymol in the ethanolic fraction revealed that the isolated constituent is the most active compound. Isolation of bioactive compound thymol may help in the identification of various pharmacological activities and carrying out further research in C. peltata.