ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF HEAVY METAL RESISTANT BACTERIA FROM SOIL OF AN IRON INDUSTRY, HARYANA (INDIA).Abstract
Metallic contaminants in the soil are hazardous and their accumulation in plants and water may be dangerous. Microorganisms have properties to adsorb, absorb and accumulate heavy metals. Moreover, they play the role of problem solver by resolving key problems associated with different kinds of metals. The study was undertaken to isolate and identify heavy metal resistant bacteria in soil around iron industries of Sonipat district in Haryana (India). Eight isolates were selected on the basis of heavy metal tolerance and antibiotic resistance in the secondary screening. Out of eight, one of the strain was authentically identified as Rhizobium halophytocola strain KT327204.1 (RT7) on the basis of morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis. The minimal inhibitory concentration (MIC) of isolate against Mn (7mM), Ni (1mM), Pb (3mM) and Fe (1mM) was determined in solid media. The identified isolate showed optimum growth at 37°C, 7.0pH and at NaCl concentration of 1% (w/v).
Richa Gupta, Tarun Kumar and Amita Mittal*
Department of Biotechnology Engineering, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra, Haryana, India
30 September, 2015
15 December, 2015
05 January, 2016
01 March, 2016