ISOLATION, IDENTIFICATION OF NON-POLAR TERPENES AND TOTAL ANTIOXIDANT ACTIVITY FROM CHLOROFORM EXTRACT OF BOUGAINVILLEA GLABRA LEAVESAbstract
This study reports the isolation and antioxidant activity of non-polar terpenes extracted from the leaves of Bougainvillea glabra choicy. To characterize the phytochemical constituents of Bougainvillea glabra leaves using the GC-MS method. The 95% ethanol extract was filtered, distilled, and concentrated to obtain the solid greenish residue. The residue was further fractioned by n-hexane, chloroform, ethyl acetate, acetone, ethanol, and methanol. The GC-MS analysis of Chloroform extract of Bougainvillea glabra leaves revealed the presence of thirteen compounds that could contribute to the medicinal quality of the plant. The first compound identified with less retention time (9.50 min) was 1-(1-methyl-decyl)-decahydro-napthalene. The isolation of organic compounds was done using column and thin layer chromatographic techniques. Compound characterization using various spectroscopic techniques identified the final isolated compound as 1-(1-methyl-decyl)-decahydronapthalene. Antioxidant activity was evaluated by the DPPH method. The method of isolation is simple, cost-effective, and efficient.
J. M. J. Rani
Department of Chemistry, Annai Vailankanni Arts and Science College, Bishop Sundaram Campus, Thanjavur, Tamil Nadu, India.
23 March 2020
08 July 2020
15 July 2020
01 March 2021