ISOLATION OF ANTIBACTERIAL PEPTIDE FROM VIGNA ACONITIFOLIA L.Abstract
Vigna aconitifolia L. represents a genus in the Fabaceae family and is one of the most drought-resistant legumes, commonly grown in arid and semi-arid regions of India. Therapeutic effects of Vigna aconitifolia L. have been reported in several publications, but there are only a few reports on the biological activities of the Vigna aconitifolia L. proteins. In the present study, antibacterial activities of the Vigna aconitifolia L., peptides have been investigated. Homogenized seed flour was pulverized, and its protein content was extracted in a phosphate buffer. After centrifugation, by adjusting pH, peptides from the supernatant were isolated. Gel filtration (G-25) and Ion-exchange chromatography on the seed extract gave fairly pure peptide fractions of which inhibitory activities were done by agar well diffusion method against Shigella flexineri, Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Enterococcus faecalis, Vibrio cholerae bacterial pathogens. The results indicate that a 30kDa of the peptide was identified and exhibited good antibacterial activity against bacterial pathogens. Among all strains, Shigella flexineri and Staphylococcus aureus exhibit good results with a clear zone of inhibition.
D. Swathi * and N. Mallikarjun
Department of Studies and Research in Microbiology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India.
05 September 2022
18 October 2022
17 November 2022
01 May 2023