ISOLATION OF COUMARIN COMPOUND FROM THE BARK OF BAUHINIA PURPUREAAbstract
Objectives: The study aimed to isolate coumarin compound from Bauhinia purpurea bark. Method: The bark was extracted successively with methanol in a soxhlet apparatus. The crude mass was subjected to HPLC, as well as column chromatography and eluted with methylene dichloride – methanol system with increasing methanol ratio to isolate compound BP. The structure of compound BP was identified by various spectrophotometric techniques (UV, IR, 1HNMR, 13CNMR and Mass Spectrometry). Key Findings: Compound BP was obtained as a white crystalline solid with melting point 197oC. It responded positively to the test of flavonoids. It showed λmax at 256 and 356 nm, IR bands at 3258.93, 1343.97, 1655.57, 1165, 1198.52 cm-1, NMR spectra confirmed the presence of phenolic OH and coumarin ring and [ESI-MS] m/z 462.9 [M+– 1]. Conclusion: Concluding from the interpretations of all the spectra, the compound BP was identified as 3, 5, 7- trihydroxy – 2 – (3, 4, 5- trihydroxy phenyl) – 4H-1 – benzopyran – 4- one. This compound had been found worthy for pharmacological considerations.
S. Pahwa *, R. Mazumder and S. Bhattacharya
Associate Professor Lloyd School of Pharmacy, Lloyd Institute of Management and Technology, 11, Knowledge Park-II, Institutional Area, Greater Noida-201306, India
16 May, 2014
17 July, 2014
16 September, 2014
01 January, 2015