ISOLATION OF ENDOPHYTIC FUNGI FROM DIPTEROCARPUS GRANDIFLORUS BLANCO AND EVALUATION OF XYLANASE ENZYME ACTIVITY FROM FERMENTATION BROTHAbstract
Xylanase enzyme, one example of secondary metabolites generated by the endophytic microbes was an enzyme which degrades Xylan. The current research study was focused on isolation of endophytic fungi from Dipterocarpus grandiflorus Blanco that produced xylanase enzyme and evaluation of this enzyme activity. Isolation of endophytic fungi from Twigs of D. grandiflorus Blanco were performed via direct seeding inoculation technique utilizing Corn Meal Malt (CMM) medium, followed by purification of these isolates in Potato Dextrose Agar (PDA) media and macroscopic observations to characterize these isolates. The presence of xylanase enzyme was determined using Dinitro Salicylic Acid (DNS) method. Over 12 days our study results yield a total of eight (8) endophytic fungi isolates with six xylanase enzyme producing isolates (Dg II,3 a2, Dg II,3 a3, Dg II,3 b1, Dg II,3 c1, Dg II,3 e1, Dg II,3 f1). Highest extracellular xylanase activity was found in Dg II, 3fl (4.036 U/mL) while the lowest one was 0.426U/mL in isolate Dg II, 3bl.
Shirly Kumala* and Anggraeni Puji Handayani
Faculty of Pharmacy, University of Pancasila. .Jakarta, Indonesia
14 August, 2010
17 November, 2010
26 December, 2010