ISOLATION OF EPICATECHIN FROM OXYSTELMA ESCULENTUMAbstract
Background: Oxystelma esculentum is used traditionally as diuretic, laxative, hepatoprotective and anti-ulcer.
Objectives: The present work deals with the isolation and structure elucidation of Epicatechin from O. esculentum.
Methods: Methanolic extract of the entire plant of O. esculentum was subjected to pilot TLC experiments and column chromatography.
Results: This yielded a pure, white compound which resolved at Rf 0.3 on TLC plate using the mobile phase toluene: ethyl acetate (5:4) when developed in iodine chamber. This compound was subjected to UV, IR and 1H-NMR spectral analysis and its structure elucidation revealed it to be Epicatechin.
Conclusion: This compound has been reported for the first time in this plant and can serve as a useful tool in its standardization.
D.J. Pandya* and I.S. Anand
Associate Professor, School of Pharmacy, RK Technical Campus, RK University, Rajkot, Gujarat, India
03 September, 2012
08 October, 2012
29 December, 2012
01 January, 2013