ISOLATION, SCREENING AND IDENTIFICATION OF UREASE-PRODUCING BACTERIAL STRAIN BACILLUS SPECIES FROM MARINE SEDIMENTS
AbstractMarine microorganisms are a valuable source of novel enzymes with ideal characteristics because of the halophilic nature of marine bacteria. The present study aims to isolate, screen, and identify the marine bacterial strains from marine sediment samples that produce an enzyme urease with clinical and industrial applications. Marine sediment samples were collected and cultured on zobell marine agar medium. After incubation seven bacterial strains were isolated from the culture. From this, five isolates were showed urease activity on the urea agar base medium. Morphological and biochemical test of urease-producing organisms was done. Urease-producing bacterial strains (UR S7B) were selected on the basis of their urease test. The selected strain UR S7B was further sequenced for identification. The phylogenetic characterization and 16S rRNA of the strain UR S7B revealed that bacterial cultures belong to Bacillus sp. The morphological studies indicated that the isolate was Gram -ve, rod-shaped and motile organism. The present study concludes that marine bacteria can be a urease source with important applications.
Article Information
37
3527-3532
703 KB
301
English
IJPSR
V. Priyasenan * and K. S. Athira
Department of Biotechnology, SAS SNDP Yogam College, Konni, Pathanamthitta, Kerala, India.
priyabiotech2021@gmail.com
02 November 2022
18 December 2022
31 December 2022
10.13040/IJPSR.0975-8232.14(7).3527-32
01 July 2023
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