LARVICIDAL EFFECT OF THE PET. ETHER, CHLOROFORM FRACTIONS AND METHANOL EXTRACT OF BUCCHOLZIA CORIACEA ENGLE SEEDAbstract
Buccholzia coriacea Engle (Capparidaceae ‘wonderful cola’) a tropical plant is used in traditional medicine in the management of diabetes, malaria and hypertension. This study aims to investigate the larvicidal activities of the Petroleum ether, chloroform fractions and methanol extract of B. coriacea seed as a potential agent in vector control for malaria. Third and fourth instar larvae of Anopheles gambiae were used for the study. Five doses (62.5 μg/ml, 125 μg/ml, 250 μg/ml, 500 μg/ml and 1000 μg/ml) of the methanol extract petroleum ether and chloroform fractions tested against the larva with 1% ethanol used as control. The mortality rates were determined 24 and 48 hr after exposure to extract following the WHO procedure. The chloroform fraction exhibited the highest mortality rate of 100% at 250, 500, 1000 μg/ml after 24 hr exposure, the pet ether exhibited mortality rate of 77.5% (1000 μg/ml) after 48 hr exposure while the methanol extract had the lowest effect of 22.5 % after 48 hr. This study reports for the first time the larvicidal activity of B. coriacea which is used in the folklore medicine as treatment for malaria.
Fred-Jaiyesimi Adediwura*, Ogbole Omonike , Anthony Olamide and Egbebunmi Oluwatosin
Department of Pharmacognosy, Olabisi Onabanjo University, P.M.B. 2022, Sagamu Campus, Ogun State, Nigeria
01 April, 2011
13 May, 2011
27 June, 2011
01 July, 2011