METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE, TENOFOVIR AND EFAVIRENZ IN COMBINED TABLET DOSAGE FORM BY RP-HPLC AND UV-SPECTROSCOPIC METHODAbstract
A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for the determination of Lamivudine, Tenofovir and Efavirenz simultaneously in combined tablet dosage form was developed. Chromatography was performed with mobile phase containing a mixture of methanol: Water (pH adjusted to 2.5) and 0.1 % TEA in the proportion of (68: 32 % v/v) the samples were injected onto Symmetry C18 Column (4.6 x 100mm, 5μm, Make: HYPERSIL ODS) column. The flow rate was 1.2ml.min–1. The samples were detected at 260nm. The UV spectrophotometric method was performed at 272nm for Lamivudine, 260 nm for Tenofovir and 247 nm for Efavirenz, and samples were prepared with a solution of Water methanol (30:70 % v/v). The assay was linear in range from 25% to 150% targeted concentration and regression coefficient for all three drugs was found to be 0.999 highly significant for the method. The proposed methods were simple, rapid, precise, accurate and sensitive, and can be used for the routine of the quality control in pharmaceuticals.
Akula Srinath*, B. Sneha, Akhila Alladi, Rayees Ahmed and R.G. Kulkarni
Department of Pharmaceutical Analysis, Trinity College of Pharmacy, Peddapally, Karimnagar, A.P., India.
16 May, 2014
19 July, 2014
17 August, 2014
01 December 2014