METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NICOTINAMIDE AND METRONIDAZOLE IN GEL USING UV-SPECTROPHOTOMETER
AbstractAn easy, precise and accurate spectroscopic technique for the simultaneous estimation of nicotinamide and metronidazole in semi-solid dosage form and in bulk form has been developed. The present method involves the addition of 0.1M hydrochloric acid solution, followed by dilution with water and subjecting the resulting solution to UV spectroscopic assessment. Absorption maximum was found to lie at about 262 nm and 317 nm for nicotinamide and metronidazole respectively. The calibration curve showed linearity as per line equation y=0.0029x + 0.0001 with an R2value of0.9937 for nicotinamide and y = 0.0072 x + 0.0063 with an R2 value of 0.9933 for metronidazole. Validation was performed as per ICH guidelines for linearity, accuracy and precision. The assay result was in a good arrangement with the label claim. The mean Sandell’s sensitivity values for metronidazole and nicotinamide, respectively, were 0.354 g/cm2 and 0.134 g/cm2, showing that the approach is very sensitive and practical for measuring both substances.
Article Information
17
1427-1432
595 KB
607
English
IJPSR
V. Likith Kumar, R. Sharveswaran, K. Saranya, S. Naresh, S. Nagoor Meeran Siraj and S. Gayatri *
Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai, Tamil Nadu, India.
gayatrisru@yahoo.co.in
11 October 2023
05 January 2024
04 April 2024
10.13040/IJPSR.0975-8232.15(5).1427-32
01 May 2024
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