METHOD DEVELOPMENT AND VALIDATION OF FOLIC ACID BY UV-VISIBLE SPECTROSCOPYAbstract
The present study a simple, accurate, precise and cost effective UV-Visible spectrophotometric method for development and validation of folic acid as vitamin. folic acid is a water soluble vitamin, so solvent used throughout the experiment was chloroform, butanol,0.1N NaoH, water .the absorption maxima of drug was found at 250nm . Beer’s law was obeyed in the range of 5ppm-30ppm. The developed method was successfully validated with respect to linearity, accuracy and precision. The method was validated and shown linearity in mentioned concentration. The percentage relative standard deviation of inter-day and intra-day precision range 45.15%. Hence proposed method was precise, accurate and cost effective, simple and rapid. This validated method can be applicable for quantitative determination of the titled drug with respect to assay for solid dosage form.
D. B. Throat *, Kalyani Pawar and Paunekar Ashwini
Department of Pharmaceutical Analysis, SND College of Pharmacy, Babhulgaon, Yeola, Nashik, (M.S.), India
28 November, 2014
10 April, 2015
21 May, 2015
01 July, 2015