METHOD DEVELOPMENT AND VALIDATION OF PARA-PHENYLENEDIAMINE IN PURE AND MARKETED DYES BY USING RP-HPLC
AbstractTo estimate the presence of para-phenylenediamine in various commercial hair colors, a straightforward, speedy, precise investigation used High-Performance Liquid Chromatography to find PPD in henna and determine its presence. In order to do the chromatography, stationary phase Phenomenox C18 100 mm x 4.6 mm, 5µm column system was used for UV detection with a242 nm wavelength. The mobile phase Methanol with water (HPLC grade) (70:30) was used as the mobile phase for the technique, which was created and validated for the detection of PPD in samples of commercial hair color. With a run time of 7 minutes, a retention time of 2.088 seconds, and a flow rate of 0.55 ml/min, the method was validated over a broad linear range of 5-28 g/ml. The PPD content of hair colors may not exceed 6%. The procedures were approved in line with ICH guidelines.
Article Information
32
571-576
636 KB
316
English
IJPSR
Khaga Bhavyasri * and Amuktha Malyada
Department of Pharmaceutical Analysis, RBVRR Women’s College of Pharmacy, Barkatpura, Hyderabad, Telangana, India.
Bhavya.khagga@gmail.com
12 June 2023
12 January 2024
28 January 2024
10.13040/IJPSR.0975-8232.15(2).571-76
01 February 2024
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