METHOD DEVELOPMENT AND VALIDATION OF TULOBUTEROL IN API AND ITS PHARMACEUTICAL DOSAGE FORMS BY UV SPECTROPHOTOMETRYAbstract
A simple, accurate, sensitive and reproducible UV spectrophotometric method has been developed for the determination of Tulobuterol (TLB) in bulk and also in its pharmaceutical dosage formulations. The proposed method showed absorbance maxima at 212 nm. Beer’s law is obeyed over a concentration range of 25-125µg/mL. The respective linear regression equation being Y =0.009x +0.014 for TLB. Results of analysis for the method established, was validated statistically and also by recovery studies. The apparent molar absorptivity and Sandell’s Sensitivity values are 0.43×104 Lmol-1cm-1 and 0.0371 µgcm-2 respectively. The assay and recovery studies were found to be 99.16% and coefficient correlation(r) was found to be 0.999. The different experimental parameters effecting the development and stability were studied carefully and optimized. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were also obtained. The developed method employed was successful for the determination of TLB in various pharmaceutical preparations.
Y. Ravindra Reddy* and K.V.M. Krishana
Department of Pharmaceutical Analysis and Quality Assurance, Teegala Ram Reddy College of Pharmacy, # 4 – 202, Meerpet, Saroonagar (M), Hyderabad– 500097, Andhra Pradesh, India
23 May, 2013
28 August, 2013
23 October, 2013
01 November, 2013