METHOD DEVELOPMENT FOR ESTIMATION OF DICLOFENAC SODIUM IN A CHOCOLATE DOSAGE FORMAbstract
A simple, rapid, precise, sensitive and reproducible Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method has been developed for the quantitative analysis of Diclofenac sodium in the chocolate dosage form. Chromatographic separation of Diclofenac sodium was achieved on waters alliance-e2695, by using waters symmetry C18, 150 mm × 4.6 mm, 3.5 µm, column and the mobile phase containing 0.1% formic acid & ACN in the ratio of 30:70% v/v. The flow rate was 1.0 ml/min; detection was carried out by absorption at 275 nm using a photodiode array detector at ambient temperature. The calibration curve was linear over the range of 5-75 µg/ml. The number of theoretical plates and tailing factor for Diclofenac sodium was NLT 2000 and were not more than two, respectively. The proposed method was validated according to ICH guidelines. The method was found to be simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Diclofenac sodium and study of its stability.
J. L. Prasanna *, A. M. S. S. Babu, C. H. N. Jyothi and T. M. Rao
A. M. Reddy Memorial College of Pharmacy, Narasaraopet, Andhra Pradesh, India.
04 April 2019
24 July 2019
12 August 2019
01 January 2020