METHODS OF EXTRACTION AND FRACTIONATION OF BIO-ACTIVE COMPONENTS OF MORINDA COREIA FOR ANTI-TUBERCULAR AND ANTICANCER ACTIVITIES

In Asian countries, roots, barks, stems, leaves, and fruits of plants belonging to the Morinda species are considered as valuable traditional herbs. They are used in folk medicine for the treatment of many diseases, including diabetes, high blood pressure, and cancer. Anti-tubercular and cytotoxic activities of Morinda citrifolia (noni) have been reported, but Morinda coreia has not been explored for these two activities to date. In the present study, a systematic investigation was carried out with the air-dried leaves of M. coreia by bio-assay guided fractionation by three different extraction methods including conventional solvent extraction (SE), microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) methods to identify the best method for extraction for bioactive fractions responsible for anti-mycobacterium and anticancer activities. The n-hexane fraction of M. coreia was found to be most with MIC values ranging from 125 µg/L for SE and to 62.5 µg/mL for UAE against Mycobacterium H37Rv. Similar results were obtained against MCF-7 cells with IC₅₀ values 33.78 µg/mL for SE and 22.22 µg/mL for UAE. In conclusion, the present study established that the most potential bioactive fraction of M. coreia can be best extracted with UAE.