MICRO-ELECTROPHORETIC MOBILITY STUDY OF THE INFLUENCE OF CHLORPROMAZINE HYDROCHLORIDE ON DIMYRSTOYLPHOSPHATIDYLCHOLINE LIPOSOMES IN DIFFERENT MEDIAAbstract
In this study, the possible influence of the model, cationic drug chlorpromazine hydrochloride (CPZ-HCl) concentration on the micro-electrophoretic mobility of neutral large multi-lamellar dimyrstoylphosphatidylcholine (DMPC) liposomes was investigated in various media at both 25oC and 37oC. In addition the effect CPZ-HCl concentration on the micro-electrophoretic mobility of both positively charged DMPC/stearyl amine (ST) liposomes and negatively charged DMPC/dicetyl phosphate (DCP) liposomes were also investigated. The effect of pH on the micro-electrophoretic mobility of neutral positively charged and negatively charged liposomes in the absence and presence of CPZ-HCl in water and in 0.1M KCl at 25oCwere also studied. Lastly, the influence of CPZ-HCl at concentrations known to cause anesthesia, on the micro-electrophoretic mobility of neutral, positively charged and negatively charged liposomes in phosphate buffer (pH 7.4) at 37oC was also investigated. Present study indicates that DMPC liposomes in water exhibited zero electrophoretic mobility over a wide pH range. DMPC liposomes acquired negative charges in 0.1M KCl, phosphate buffer (pH 6.0 and 7.4) and in human plasma (pH 7.4). The addition of CPZ-HCl shifted the mobility of neutral, positive and negative charged DMPC liposomes towards positivity. Linear relationship were observed between the electrophoretic mobility of DMPC, DMPC /ST, and DMPC /DCP liposomes as a function of CPZ-HCl concentrations known to cause anesthesia.
Farah Hamad Farah
Department of Pharmaceutics, College of Pharmacy and Health Sciences, Ajman University of Science and Technology, Ajman PO Box 346, United Arab Emirates.
26 December, 2014
12 March, 2015
28 May, 2015
01 August, 2015