MOLECULAR DOCKING AND BIOLOGICAL EVALUATION OF ACACIA FERRUGINEA AND ITS ACTIVE PRINCIPLE, SEVERIN, AGAINST CHROMOBACTERIUM VIOLACEUMAbstract
Misuse and overuse of antibiotics, multi drug resistance bacterial strains have emerged as silent pandemic. Lately, antibiofilm treatment approach using natural compounds have ascended as an alternate strategy to overcome the issue. In this study, the inhibitory effect of bark and leaf extracts of Acacia ferruginea against a model bacterium, Chromobacterium violaceum was evaluated. Results revealed that A. ferruginea extract has a significant inhibitory effect on virulence determinants (biofilm and violacein productions) with no effect on growth.19 unrelated compounds were identiﬁed from GC-MS analysis. In molecular docking studies, six hits were found to have potential interactions with CviR, a quorum sensing regulatory protein. One of the major hits, severin, formed H-bond with Ser155 and Asp 97 with highest Gscore -8.862 when compared to the standard ligand, C6HSL (-7.052). Our data showed that the leaf and bark ethanolic extracts of A. ferruginea will be a promising synergetic remedy against bacterial infections from natural sources by curbing quorum sensing.
M. Jeevitha *, Vinothkannan Ravichandran and Shubashini K. Sripathi
Department of Chemistry, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, India.
18 August 2018
28 December 2018
17 January 2019
01 April 2023