NANONEEDLE MEDIATED NONINVASIVE AND PAINLESS TRANSDERMAL DELIVERY OF INSULINAbstract
We have explored the nanoneedles from nanofabrication techniques, which could penetrate both nuclear and cellular membranes of cells. We received the individual nanoneedles with a tip diameter of 220-250 nm and the desired microscale length ~1 to 100 μm, which enable them for transdermal delivery of selective drug molecule by piercing stratum corneum, the outermost layer of skin. These nanoneedles possess Young’s modulus ~100 GPa, which is necessary for mechanical stability of nanoneedles during its penetration across stratum corneum for transdermal drug delivery. On the prefabricated structure of silicon, the arrays of nanoneedles were grown. The nanoneedles were further bio-functionalised using conjugation of drug molecule like Insulin with PEG and successfully delivered into the rat’s skin. The nanoneedles could be act as a vehicle for transport of Insulin drug molecules. The surface morphologies, basic structure and composition of the nanoneedles were confirmed using SEM, TEM, FTIR, ICPAES, XRD and TGA. Super-conducting quantum interference device (SQUID) magnetometer was used to ascertain the magnetic properties of nanoneedles. In-vivo studies after administration of insulin by nanoneedles exhibited rapid decline in blood glucose levels in diabetic rats. It may use as a nascent contrivance for a noninvasive and painless transdermal delivery of insulin.
B. S. Wakure * and N. M. Bhatia
Vilasrao Deshmukh Foundation, Group of Institutions, VDF School of Pharmacy, Latur, Maharashtra, India.
26 December 2019
31 March 2020
05 April 2020
01 January 2021