NEW MICROBIAL SOURCES OF PUFA FROM THE ARABIAN SEA AND FRESH WATER HABITATS OF INDIAN TERRITORYAbstract
Polyunsaturated Fatty Acids (PUFA) have been shown to be of major importance in the prevention and treatment of a range of human diseases and disorders. A number of microorganisms have been shown to produce and accumulate PUFA. In the current study we have isolated 86 lipid producing microbes from various sources like a river from northern India, lakes from southern India and marine environments off the western coast of India. The isolates were shortlisted following a preliminary screening scheme by studying a set of parameters like intensity of lipid accumulation as judged by Sudan Black B staining, amount of biomass on nitrogen limited semi-synthetic medium, lipid quantity per gram biomass, relative quantities of saturated and unsaturated fatty acids and reproducibility of results. Lipid sample was extracted and derivatized for GC analysis using standard procedure. Further, GC/MS analysis confirmed that 5 isolates produce PUFA of omega-6(LA) and omega-3(ALA, ETA) type. Selected cultures have been identified by 16S r-RNA sequencing and are being optimized for PUFA production by the technique of fermentation
A. A. K. Masurkar, A. G. Datar and B. V. Vakil
Guru Nanak Institute for Research and Development (G.N.I.R.D), GN Khalsa College, Matunga, Mumbai 400 019, Maharashtra, India.
27 May, 2014
26 July, 2014
20 September, 2014
01 January, 2015