NOVEL, RAPID, ISOCRATIC RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF BETAMETHASONE DIPROPIONATE, MICONAZOLE NITRATE AND CHLOROCRESOL IN PHARMACEUTICAL DOSAGE FORMAbstract
A novel, rapid, isocratic, reversed-phase HPLC method was developed to simultaneously determine betamethasone dipropionate, miconazole nitrate and chlorocresol in a topical dosage form. The separation was performed on the C18 prontosil (250 x 4.6mm x 5µ) column. The mobile phase used for this study consists of acetonitrile and phosphate buffer of pH 2 in the ratio of 70:30 v/v, respectively. The flow rate was set as 1 ml per minute. This method used a UV detector with a wavelength of 235 nm. The retention time of chlorocresol, miconazole nitrate, and betamethasone dipropionate was 4.30 min, 5.13min, and 6.49 min, respectively. Linearity was observed for betamethasone dipropionate, miconazole nitrate, and chlorocresol in the concentration range of 1 to 3 µg/ml, 40 to 120 µg/ml and 2 to 6 µg/ml, respectively. The novel, rapid, reversed-phase HPLC method can be used in the regular analysis of any drug or combination in the pharmaceutical dosage form.
Vandana Jain * and Pallavi Gadhave
Department of Quality Assurance, Oriental college of Pharmacy, Sanpada, Navi Mumbai, Maharashtra, India.
30 May 2022
16 July 2022
03 August 2022
01 February 2023