OPTIMIZATION AND EVALUATION OF ANTIMICROBIAL COMPOUNDS PRODUCED BY STREPTOMYCES RUBRUS VLK-24Abstract
Optimizing the cultural conditions to enhance bioactive metabolite production by Streptomyces rubrus VLK-24 isolated from mangrove sediments as well as characterizing these compounds, has been taken up in this study. The culture broth inoculated in Yeast extract, Malt extract, and Dextrose broth and extracted with ethyl acetate exhibited high antimicrobial activity against test microorganisms, including Gram-negative and Gram-positive bacteria as well as fungi. The crude ethyl acetate extract exhibiting high antimicrobial activity was analyzed by Gas Chromatography-Mass Spectroscopy, which evidenced the presence of 39 compounds according to the available library data, NIST MS Search (ver. 2.0). The results of the study revealed the production of diversified metabolites by the strain; hence it could be a possible source for novel antimicrobial compounds.
Krishna Naragani * Mani Deepa Indupalli, B. Sarath Chandra and K. V. S. Durga Prasad
Department of Botany, Hindu College, Market Road, Guntur, Andhra Pradesh, India.
24 August 2022
11 April 2023
18 April 2023
01 May 2023