OPTIMIZATION AND VALIDATION OF A METHOD FOR THE QUANTIFICATION OF POLYSACCHARIDES OF THUJA OCCIDENTALIS LINN.Abstract
The anthrone method has been employed for many years in the spectrophotometric determination of polysaccharides. However, taking into account that its use usually involves a series of steps and requires a long period of analysis, this study aimed to conduct a more detailed assessment of its application in the determination of total polysaccharides from the aerial parts of Thuja occidentalis Linn. Thus, this study attempted to optimize and validate a viable method for this species, whose antimetastatic and immunostimulant properties have been attributed to the metabolites analyzed in this study. The optimized and validated method showed satisfactory results, and this method lasts about 6 hours, in contrast to the diverse methodologies reported in the literature with an estimated duration of 41 hours. Thus, the presented method demonstrated to be a viable tool for the quality control of the plant drug and products derived from the aerial parts of T. occidentalis.
G.S. Marques, C.C.A.R. Silva, W.T. Vilela, C.A.A. Silva, G.C.R. Medeiros, R.M.F. Silva, P.J. Rolim-Neto*
Laboratory of Medicine Technology, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Professor Artur de Sá Avenue, Cidade Universitária, Recife, PE, ZIP: 50740-520, Brazil
11 February, 2014
17 April, 2014
15 June, 2014
01 August, 2014