OPTIMIZATION OF CULTURAL CONDITIONS TO ENHANCE BIOACTIVE METABOLITE PRODUCTION BY STREPTOMYCES OLIVOVERTICILLATUS VLK-15Abstract
The study was designed to optimize cultural conditions for enhanced production of bioactive compounds by Streptomyces olivo-verticillatus VLK-15 isolated from mangrove ecosystem of South Coast of Andhra Pradesh, India. The soil dilution plate technique was employed for the isolation of actinobacteria using starch-casein agar (ISP-6) medium. The potent strain was identified as Streptomyces olivoverticillatus VLK-15 based on polyphasic taxonomy. The productivity of the strain was enhanced by amending the medium (ISP-2) with mannitol (0.5%), peptone (0.5%), and K2HPO4 (0.05%) adjusted to pH 7.0 and incubated with the culture at 30ºC for ninety-six hours. The secondary metabolites produced under optimized conditions exhibited high antimicrobial activity against Gram-positive and Gram-negative bacteria as well as fungi. Hence, it could be a possible source for novel and potential antimicrobial compounds.
Krishna Naragani *, Mani Deepa Indupalli and Vijayalakshmi Muvva
Department of Botany, Hindu College, Guntur, Andhra Pradesh, India.
04 February 2021
04 May 2021
, 29 May 2021
01 December 2021