OPTIMIZATION OF INFLUENZA A AND B VIRUSES CULTIVATION CONDITIONS FOR PREPARATION OF TRIVALENT SEASONAL INFLUENZA SPLIT VACCINEAbstract
This article presents the results of studies on determination of optimal parameters of recombinant strains A/NYMC X-217 (H3N2) and B/NYMC BX-49 of influenza virus cultivation in chicken embryos. It is found that a maximum virus accumulation is formed under the following cultivation conditions: age of chicken embryos is 9-11 days in case of inoculation of the allantoic cavity, inoculated virus dose — 1,000-10,000 EID50, temperature and time of incubation — (34 ± 0.5) ºС and 48 h, respectively. If the cultivation parameters are satisfied, it is possible to obtain a highly active virus-containing material with infectious and hemagglutinating activity, as well as a content of hemagglutinin not less than 7.0lg EID50/ml, 1:256 and 7.0μg/ml, respectively, that is enough to prepare a trivalent inactive seasonal influenza split vaccine.
I. Akzhunusova *, N. Assanzhanova , K. Tabynov , B. Abdrakhmanova and A. Sansyzbay
The Research Institute for Biological Safety Problems , Zhambulskaya oblast, Kordaiskiy rayon, Gvardeisky, Republic of Kazakhstan
15 October, 2014
15 December, 2014
08 February, 2015
01 June, 2015