OPTIMIZATION OF SOME CULTURE CONDITIONS FOR IMPROVED BIOMASS AND ANTIBIOTIC PRODUCTION BY STREPTOMYCES SPECTABILIS ISOLATED FROM SOILAbstract
A Streptomyces strain was isolated from soil and was identified as Streptomyces spectabilis on the basis of 16 S rRNA gene sequencing. On screening for its ability to produce antimicrobial compounds, it was found to be active only against Gram positive bacteria. Attempts were made to optimize the culture conditions for the production of antimicrobial compounds. Basal medium supplemented with glucose as a carbon source was found to be the best for growth but not for antibiotic production. Antibiotic production proved to be highest in the medium amended with cellobiose. Jack bean meal (JBM) and peptone were the best nitrogen sources for growth and antibiotic production respectively. Antibiotic production appeared to start after 48 hours and reached a maximum on the fourth day. Maximum growth and antibiotic production occurred when the initial pH was adjusted to 5.
S.K. Holkar*, D.N. Begde , N.A. Nashikkar , T.A. Kadam , A.A. Upadhyay 2
School of Life Sciences, SRTMU , Nanded-431 401, Maharashtra, India
22 March, 2013
02 May, 2013
21 July, 2013
01 August, 2013