OPTIMIZATION STUDIES FOR ENHANCED BIOACTIVE METABOLITE PRODUCTION BY STREPTOMYCES VIOLACEORUBER VLK-4 ISOLATED FROM THE SOUTH COAST OF ANDHRA PRADESH, INDIAAbstract
The aim of the present study was to design the suitable culture medium and also optimize the culture conditions for enhanced production of antimicrobial metabolites by Streptomyces violaceoruber VLK-4 which exhibited a broad spectrum of activity against bacteria and fungi. Production of bioactive metabolites by the strain was high in modified yeast extract-malt extract dextrose (ISP-2) broth as compared to other media tested. Mannitol (0.4%) and asparagine (1%) were found to be the most suitable carbon and nitrogen sources for the optimum production of bioactive metabolites as well as growth. Maximum production of bioactive metabolites was found in the culture medium with an initial pH 7.0 incubated for five days at 30 ºC under shaking conditions. This is the first report on the optimization of bioactive metabolites by Streptomyces violaceoruber VLK-4.
K. Naragani, R. K. Munaganti and V. Muvva *
Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
15 April 2014
08 July 2014
30 July 2014
01 November 2014