ORTHOSIPHON STAMINEUS INCREASES METABOLISM OF AMINOPYRINE IN FEMALE RAT HEPATOCYTES BY INHIBITING PROTEIN KINASE- AAbstract
Orthosiphon stamineus (Family: Lamiaceae) has becoming one of the popular medicinal plants in Malaysia due to its high beneficial effects in treating diabetes mellitus, hypertension and kidney stones. The present study was conducted to evaluate the mechanism of O. stamineus in affecting metabolism of aminopyrine in female rat hepatocytes via second messenger pathway. Liver perfusion was used to isolate hepatocytes from the healthy female Sprague Dawley (SD) rats. Isolated perfused rat hepatocytes were pretreated with several enzyme inhibitors/stimulant targeted on the protein kinases, phosphatase and phosphodiesterase. From the results obtained, only the activity of aminopyrine N-demethylase in female rat hepatocytes pre-treated with KT5720 showed significantly increased in the presence of 1 ng/ml (P<0.05) of O. stamineus extract. For conclusion, 1 ng/ml of O. stamineus extract increased the metabolism of aminopyrine in rat hepatocytes by inhibiting the protein kinase- A activity.
Jin Han Chin and Abas Haji Hussin
Senior Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, UCSI University 1, No.1, Jalan Menara Gading, 56000, Kuala Lumpur, Malaysia
08 April, 2011
11 May, 2011
26 June, 2011
01 July, 2011