PHARMACOGENOMIC STUDIES OF CHOLESTERYL ESTER TRANSFER PROTEIN (CETP) GENOTYPES IN SUSPECTED CAD PATIENTSAbstract
Recent studies have attempted to identify specific genes that contribute to cardiovascular health and disease. Response to various drugs like statins differs from individual to individual, due to polymorphic variations in drug metabolizing genes related to pharmacogenomics of the diseases. The aim of this study was to find out the association of Cholesteryl ester transfer protein (CETP) gene polymorphism rs708272 (Taq1B) with cardiovascular diseases, since variations in the CETP gene alters the HDL-C levels. The study comprises of 100 samples from individuals visiting the hospital for complaints of CVD. The Taq 1 B polymorphism was analyzed by PCR-RFLP method followed by agarose gel electrophoresis. A Taq 1 B polymorphism of CETP gene at intron 1, resulted in C to T transition. This transition induced the variations in the levels of its expression leading to altered phenotypes in the subjects. We found that CT genotypes of CETP gene were associated with higher expression levels as compared to TT and CC genotype. It is concluded that screening of Taq1B polymorphism may help to predict the prognosis of cardiovascular diseases and also their response to statins and other cholesterol lowering drugs.
Javeed Ahmad Tantray , Y. Shiva Kumar and Kaiser Jamil*
Genetics Department, Bhagwan Mahavir Medical Research Centre, 10-1-1, Mahavir Marg, AC guards, Hyderabad- 500 004, Andhra Pradesh, India
01 June, 2013
03 July, 2013
26 September, 2013
01 October, 2013