PHARMACOLOGICAL POTENTIAL OF POLYHERBAL COMBINATION FOR THE TREATMENT OF GLOMERULONEPHRITIS IN ALBINO RATS

The polyherbal combination was prepared by using aqueous and methanol root extracts of A. officinalis, B. diffusa, C. papaya, C. fistula, C. intybus, F. hispida, F. indica, C. nurvala, S. virgaurea and V. negundo in equal proportion for the dose of 25 mg/kg and 50 mg/kg body weight. Albino Wistar rats were selected for the study; the serum uric acid, urea, creatinine, BUN, and albumin level were measured using standard procedures. The elevated parameters in serum shows the statistically significant (^**p<0.05) improvement with a low dose of aqueous extract combination i.e., hemoglobin (11.15 g/dl), CBC (5.93 ×10³/µl), total bilirubin (0.61 mg/dl), total protein (09.85 mg/dl), AST (63.41 IU/L) & ALT (34.37 IU/L), uric acid (1.68 mg/dl), urea (57.06 mg/dl), creatinine (1.08 mg/dl), BUN (41.50 mg/dl) and albumin (40.84 mg/dl) in serum and urine. It was also reported as 24h urinary protein (2.96 mg/24h), albumin (09.23 mg/dl), urea (2.80 mg/dl), creatinine (38.28 mg/dl) and uric acid (126.08 mg/dl) shows significant improvement against disease control at the end of the study. Histological changes show that the gentamicin significant damaged tubular necrosis, epithelial cells, glomerular damage, and congestions of the nephron, which was completely reversed with aqueous extract polyherbal combination. Finally, it was concluded that the low dose (25 mg/kg) of aqueous extract has immense potential as compare to methanol extract and possess protective with therapeutic properties in the treatment of glomerulonephritis.